fitc cd4  (Bio-Rad)

Journal: International Journal of Molecular Sciences

Article Title: Enhanced ROS Production and Mitochondrial Metabolic Shifts in CD4 + T Cells of an Autoimmune Uveitis Model

doi: 10.3390/ijms252111513

Figure Lengend Snippet: CD4 + T cells from ERU cases produced higher levels of reactive oxygen species (ROS) after stimulation with pokeweed mitogen (PWM). Bar graphs display the factor of ROS production in CD4 + T cells from healthy horses (Ctr, gray, n = 6) and equine recurrent uveitis cases (ERU, red, n = 5) after 90 min of stimulation with PWM. The mean value is shown as a factor and the error bars correspond to the standard deviation. The factor of ROS production in control cells was determined at 3.6, while ERU cases exhibited a factor of 6.1. To compare the factor of ROS production between CD4 + T cells from ERU cases and healthy control cells, Student’s t -test was used (* p = 0.023).

Article Snippet: The purity of the CD4 + T cell fraction was tested via flow cytometry after staining of 2 × 10 5 cells of each fraction with 30 μL mouse anti-horse CD4 FITC labeled antibody (clone MCA1078F, Biorad, Feldkirchen, Germany, 1:10), which resulted in a mean purity of 98% ± 1.7% SD. ( ).

Techniques: Produced, Standard Deviation, Control

Journal: International Journal of Molecular Sciences

Article Title: Enhanced ROS Production and Mitochondrial Metabolic Shifts in CD4 + T Cells of an Autoimmune Uveitis Model

doi: 10.3390/ijms252111513

Figure Lengend Snippet: Lower dependency on and higher flexibility for mitochondrial oxidation of glucose in ERU cases. Bar graphs show the percentage of dependency on mitochondrial glucose oxidation in CD4 + T cells from healthy controls (gray, n = 6) and ERU cases (red, n = 5) as well as capacity and flexibility from healthy controls (gray, n = 5) and ERU cases (red, n = 5). The mean value is presented as a percentage, and the error bars correspond to the standard deviation. Both groups showed 100% capacity to use glucose for mitochondrial oxidation (ns = not significant), whereas CD4 + T cells from ERU cases were significantly (** p = 0.009) less dependent and exhibited significantly (* p = 0.012) greater flexibility to use glucose as a mitochondrial fuel compared to controls. Student’s t -test was used to test for statistical significance.

Article Snippet: The purity of the CD4 + T cell fraction was tested via flow cytometry after staining of 2 × 10 5 cells of each fraction with 30 μL mouse anti-horse CD4 FITC labeled antibody (clone MCA1078F, Biorad, Feldkirchen, Germany, 1:10), which resulted in a mean purity of 98% ± 1.7% SD. ( ).

Techniques: Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Enhanced ROS Production and Mitochondrial Metabolic Shifts in CD4 + T Cells of an Autoimmune Uveitis Model

doi: 10.3390/ijms252111513

Figure Lengend Snippet: Mitochondrial fuel utilization of glutamine did not show significant differences in CD4 + T cells from ERU cases. Bar graphs show the percentage of capacity and flexibility of mitochondrial glutamine oxidation in CD4 + T cells from healthy controls (gray, n = 6) and ERU cases (red, n = 5). Flexibility was calculated by subtracting the dependency from the capacity (flexibility% = capacity% − dependency%). The mean value is shown as a percentage and the error bars correspond to the standard deviation. Student’s t -test revealed no statistically significant (ns p = 0.066) differences in capacity or flexibility of substrate utilization of glutamine.

Article Snippet: The purity of the CD4 + T cell fraction was tested via flow cytometry after staining of 2 × 10 5 cells of each fraction with 30 μL mouse anti-horse CD4 FITC labeled antibody (clone MCA1078F, Biorad, Feldkirchen, Germany, 1:10), which resulted in a mean purity of 98% ± 1.7% SD. ( ).

Techniques: Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Enhanced ROS Production and Mitochondrial Metabolic Shifts in CD4 + T Cells of an Autoimmune Uveitis Model

doi: 10.3390/ijms252111513

Figure Lengend Snippet: CD4 + T cells from ERU cases showed greater capacity and flexibility to utilize long-chain fatty acids (LCFAs) for mitochondrial energy production. Bar graphs represent the percentage of capacity and flexibility for mitochondrial oxidation of LCFAs in CD4 + T cells from healthy controls (gray, n = 6) and ERU cases (red, n = 5). Flexibility was calculated by subtracting the dependency from the capacity (flexibility% = capacity% − dependency%). The mean value is shown as a percentage and the error bars correspond to the standard deviation. ERU cases showed significantly (* p = 0.041) higher capacity and flexibility to use LCFAs for mitochondrial oxidation. Student’s t-test was used to test for statistical significance.

Article Snippet: The purity of the CD4 + T cell fraction was tested via flow cytometry after staining of 2 × 10 5 cells of each fraction with 30 μL mouse anti-horse CD4 FITC labeled antibody (clone MCA1078F, Biorad, Feldkirchen, Germany, 1:10), which resulted in a mean purity of 98% ± 1.7% SD. ( ).

Techniques: Standard Deviation